tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity
trna (n(6)-l-threonylcarbamoyladenosine(37)-c(2))-methylthiotransferase activity
Catalysis of the reaction: N(6)-L-threonylcarbamoyladenosine(37) in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+.