tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase activity
trna 2-(methylsulfanyl)-n(6)-isopentenyladenosine(37) hydroxylase activity
Catalysis of the reaction: 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + acceptor-H2 + O2 = N(6)-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine(37) in tRNA + acceptor + H2O.