5-hydroxyuridine(34) in tRNA + S-adenosyl-L-methionine = 5-methoxyuridine(34) in tRNA + S-adenosyl-L-homocysteine + H(+)
5-hydroxyuridine(34) in trna + s-adenosyl-l-methionine = 5-methoxyuridine(34) in trna + s-adenosyl-l-homocysteine + h(+)