(S)-tetrahydrocolumbamine + reduced [NADPH--hemoprotein reductase] + O2 = (S)-canadine + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)
(s)-tetrahydrocolumbamine + reduced [nadph--hemoprotein reductase] + o2 = (s)-canadine + oxidized [nadph--hemoprotein reductase] + 2 h2o + h(+)